Vitexin attenuates smoke inhalation induced acute lung injury in rats by inhibiting oxidative stress via PKC β/p66Shc signaling pathway

Purpose: To investigate the protective effect of vitexin on smoke inhalation-induced acute lung injury (SI-ALI), and underlying mechanism action.Methods: The ALI rat model was established by inhalation in a closed chamber. Survival rate, arterial blood gas analysis, wet-to-dry weight ratio tissues, bronchoalveolar lavage fluid protein concentration, tissue histology, oxidative stress inflammation level were evaluated. Expressions kinase C β (PKC β), p66Shc, phosphorylated p66Shc determined western blot or quantitative reverse transcription-polymerase chain reaction.Results: Compared with group, alleviated decline partial pressure oxygen (p < 0.05), reduced exudation pathological damage, inhibited expression PKC β/p66Shc signaling pathway proteins, downregulated inflammation, ultimately improved survival rate SI-ALI rats 0.05).Conclusion: Vitexin attenuates alleviating via inhibition pathway. Thus, this compound is potential agent for treatment SI-ALI.